CE, ISO9001 Approved Eef2K (phospho-S366) Polyclonal Antibody

Model NO.
BS4060
Host
Rabbit/Mouse
Reactivity
Human, Mouse, Rat
Certification
CE, ISO9001
Transport Package
Refrigerated Transport
Specification
100ug/1mg
Trademark
Bioworld
Origin
China
HS Code
300219000
Production Capacity
3000 Vials
Reference Price
$ 222.75 - 247.50
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Product Description

Catalog No. BS4060
Product Name eEF2K (phospho-S366) polyclonal antibody
Applications WB IHC IP
Alternative Name Elongation factor 2 kinase; eEF-2 kinase; eEF-2K; Calcium/calmodulin-dependent eukaryotic elongation factor 2 kinase; EEF2K
Swiss-Prot O00418
Host Rabbit
Reactivity Human
Application_all WB: 1:500~1:1000 IHC: 1:50~1:200 IP: 1:10~1:100
Product Rabbit IgG, 1mg/ml in PBS with 0.02% sodium azide, 50% glycerol, pH7.2
Purification&Purity The antibody was affinity-purified from rabbit antiserum by affinity-chromatography using epitope-specific immunogen and the purity is > 95% (by SDS-PAGE).
Storage&Stability Store at 4°C short term. Aliquot and store at -20°C long term. Avoid freeze-thaw cycles.
Specificity p-eEF2K (S366) polyclonal antibody detects endogenous levels of eEF2K protein when phosphorylated at Ser366.
BiowMW ~ 105 kDa
Note For research use only, not for use in diagnostic procedure.
Immunogen Synthetic phosphopeptide derived from human eEF2K around the phosphorylation site of Serine 366.

  • Western blot (WB) analysis of p-eEF2K (S366) pAb at 1:1000 dilution Lane1:MCF-7 whole cell lysate(40ug) Lane2:Hela whole cell lysate(40ug) Lane3:A375 whole cell lysate(40ug)

  • Immunohistochemistry (IHC) analyzes of p-eEF2K (S366) pAb in paraffin-embedded human breast carcinoma tissue at 1:100.

Background

eEF2K is 105 kDa protein that is detected in skeletal muscle extracts and is phosphorylated rapidly by SAPK4, but poorly by p38, p38γ, JNK or ERK 2. SAPK4 phosphorylates eEF2K at Ser 359 and Ser 396 in vitro, causing its inactivation. The phosphorylation of eEF2K at Ser 359 is also induced by insulin-like growth factor-1. Ser 359 is in close proximity to Ser 366 and the Ser 366 residue also becomes phosphorylated in response to growth factors. eEF2K is phosphorylated by p70 S6 kinase at Ser 366 and this results in the inactivation of eEF2K, especially at low (micromolar) calcium concentrations. CE, ISO9001 Approved Eef2K (phospho-S366) Polyclonal Antibody CE, ISO9001 Approved Eef2K (phospho-S366) Polyclonal Antibody



CE, ISO9001 Approved Eef2K (phospho-S366) Polyclonal Antibody CE, ISO9001 Approved Eef2K (phospho-S366) Polyclonal Antibody CE, ISO9001 Approved Eef2K (phospho-S366) Polyclonal Antibody CE, ISO9001 Approved Eef2K (phospho-S366) Polyclonal Antibody CE, ISO9001 Approved Eef2K (phospho-S366) Polyclonal Antibody CE, ISO9001 Approved Eef2K (phospho-S366) Polyclonal Antibody CE, ISO9001 Approved Eef2K (phospho-S366) Polyclonal Antibody

 

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PNEUTEC.IT, 2023