Description |
Flag Tag ( DYKDDDDK Tag ) mouse monoclonal antibody |
Synonyms |
DDDDK tag, DYKDDDDK, ddk, DDDDK epitope tag, ECS epitope tag, Enterokinase Cleavage Site tag, FLAG tag, FLAG |
Immunogen |
Synthetic peptide : DYKDDDDK |
Isotype |
IgG |
Purity |
Affinity purification |
Applications |
Dilution |
Positive Tissue |
WB |
1: 2000-1: 5000 |
Flag-Tag fusion protein |
The DYKDDDDK(FLAG) peptide has been used extensively as a general tag in expression vectors. This peptide can be expressed and detected with the protein of interest as an amino-terminal or carboxy-terminal fusion. N-terminal DDDDK vectors provide an Ek cleavage site for removal of the fusion tag. The DDDDK peptide is likely to be located on the surface of a fusion protein because of its hydrophilic nature. As a result, the DDDDK peptide is more likely to be accessible to antibodies. A DDDDK-tag can be used in many different assays that require recognition by an antibody, such as western blotting, immunocytochemistry, immunoprecipitation, flow cytometry, protein purification, and in the study of protein-protein interactions, cell ultrastructure, and protein localization and so on.
Storage | Store at -20 ºC for one year. Avoid repeated freeze/ thaw cycles. |
Storage Buffer | PBS with 0.02% sodium-azide, 100 μg/ml BSA and 50% glycerol. |
Antibodies | Classification | Product Types |
Primary Antibody | ... | |
Secondary Antibody | ... | |
Loading Control Antibody |
beta Actin
GAPDH Cytoplasm / Whole Cell Mitochondria Nucleus Cell membrane Cytoskeleton |
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Tag Antibody | ... | |
Control Antibody | ... | |
Modified Antibodies | ... | |
Recombinant Antibody | ... |