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Chemical Reagent
Chaps Buffer Preparation Non Denaturing Zwitterion Detergent for Membrane Biochemistry
Model NO.
DS0174
Content
Purity Above 99%
Usage
Laboratory Reagents, Diagnostic Reagents
Source
Dry Powder
Habit Appellation
Chaps Buffer
Application
Scientific Research, Health
Property
Biochemical Reagent
Water Solubility
Soluble
Molecular Weight
614.88
Purity
Above 99%
Storage Conditions
Room Temp
Form
Powder
Colour
White
Transport Package
Carton
Specification
500g/bottle
Trademark
DeSheng
Origin
Ezhou City, Hubei Province, China
Production Capacity
5 Tons Per Month
Reference Price
$ 300.60 - 475.20
Contact Us
Description
Product Description
Packaging Specification : Plastic bottles, 500g/bottle
Basic Information
CAS Number
75621-03-3
Molecular Formula
C32H58N2O7S
Molecular Weight
614.88
EINECS
616-246-2
Appearance
White Powder
Density
1.01g/cm3
Melting Point
156-158ºC
Solubility
H2O: 50 mg/mL at 20 °C, clear, colorless
Biological activity
CHAPS, a derivative of Cholic acid, is a zwitterion detergent that can dissolve membrane proteins. CHAPS is used to stabilize various protein DNA complexes and retain the biochemical activity of proteins in solution.
In vitro research
CHAPS(0.5%)isabletostabilizecomplexesbetweenDNAandDNA-bindingfactorssuchasAP-1,SPI,GATA-1andα-regulatedfactorISGF3,Chemicalbookandretainstheirbiochemicalactivity.CHAPScanpreventdissociationofmononucleosomesdilutedtosub-nanomolarconcentrations.
Use to
CHAPS is a non denaturing zwitterion detergent used in membrane biochemistry. It can be used to dissolve membrane proteins and interrupt protein-protein interactions. CHAPS has a low micelle molecular weight (6150) and a high critical micelle concentration (6-10mM), so it can be removed from the sample through dialysis. Chemicalbook is also suitable for dissolving proteins in isoelectric focusing and two-dimensional electrophoresis. CHAPS is commonly used for non denaturing (urea free) isoelectric focusing and has been found to exhibit excellent resolution for certain subcellular preparations and plant proteins. Usually, the concentration between 2-4% (w/v) is used for isoelectric focusing gel.
Company Introduction
Hubei New Desheng Material Technology Co., Ltd. specializes in the production of blood collection tube additives, chemiluminescent reagents, biological buffers, chromogenic substrates, enzyme preparations, antigen antibodies and other biochemical reagents. Founded in 2005, Desheng has a professional R&D team and advanced production equipment!
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