5 X SDS-Page Protein Loading Buffer (odorless, reduced type) 1ml

Model NO.
G2075-1ML
Usage
Laboratory Reagents
Application
Industry, Scientific Research
Property
Biochemical Reagent
Storage
-20ºC
Validity Period
12 Months
MOQ
1 Set
Detail Usage
Protein Extraction and Denaturation
Transport Package
Carton
Specification
100ML
Trademark
Servicebio
Origin
China
Reference Price
$ 1.80 - 2.70
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Product Description

Product Description
This product uses substanceswith reducing properties similar  to  dithiothreitol (DTT) or mercaptoethanol (with Irritating odor), it is odorless and more stable in water solubility.  There  is no obvious difference significant difference from conventional SDS-PAGE protein loading buffer , so the operation of protein loading is safer and healthier.

Storage and transportation: wet ice transportation; storage at -20°C is valid for at least one year.

Composition
Component G2075 -1ml G2075 -100ml
5 x SDS-PAGE protein loading buffer (odorless, reduced type) 1mL 100ml

Steps
1. Dissolve 5×SDS-PAGE protein loading buffer in   room temperature  or 37°C   water bath before use, and store it at room temperature immediately after dissolving in the water bath. Try to avoid placing it in a water bath for a long time, and store it at -20°C after use;

2. Add the corresponding volume according to the ratio of protein sample: 5×SDS-PAGE protein loading buffer=4:1. Note: This product can also be directly used for the lysis of cell or tissue samples after being diluted to 1×;

3. Heat in a water bath or metal bath at 95°C for 10 min to fully denature the protein;

4. The samples can be loaded directly after cooling to room temperature;

5. Normally, electrophoresis can be stopped until the blue staining kits reaches the bottom of the gel.

Precautions
1. This product must be completely dissolved before usage . Store at -20°C as soon as possible after us age.
2. Avoid direct touching of  the reagents with the skin.
3. Please wear lab coat and disposable gloves during operation.

5 X SDS-Page Protein Loading Buffer (odorless, reduced type) 1ml 5 X SDS-Page Protein Loading Buffer (odorless, reduced type) 1ml 5 X SDS-Page Protein Loading Buffer (odorless, reduced type) 1ml 5 X SDS-Page Protein Loading Buffer (odorless, reduced type) 1ml 5 X SDS-Page Protein Loading Buffer (odorless, reduced type) 1ml

 

Chemical Reagent

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