Molecular Biology Grade Tris Hydrochloride (TRIS-HCL) is used in a number of Tris buffer products used in electrophoresis in the pH range 7.2-9.0. It does not precipitate calcium salts and useful in maintaining the solubility of manganese salts.
DNase, RNase,protease: none detected
CAS No: 1185-53-1
Specification
M.W. (tris base / tris HCl) 121.1 / 157.6 Purity > 99.8% Moisture < 1.0% A280 (1.0M, water) < 0.05 Insolubles < 0.005% Arsenic < 0.0005% Copper < 0.0001% Iron < 0.0001% Magnesium < 0.0001% Heavy metals (as Pb) < 0.0001% Storage Room Temperature, dry
Qty 1Kg
Tris(hydroxymethyl)aminomethane hydrochloride, 99+%
Tris, Hydrochloride is commonly used as a buffer component of phenol extraction of DNA or RNA and a buffering component of separating and stacking gels in the characterization of protein products by SDS-PAGE. Tris, Hydrochloride has also been used with urea as a method for antigen retrieval in immunohistochemistry. In smooth muscles Tris, Hydrochloride has been observed to inhibit motor responses to adrenergic motor nerve stimulation. In the crystalization of porcine pancreatic elastase with use of Tris, Hydrochloride, the Tris, Hydrochloride buffer was observed to induce conformational change and crystal-packing contraction. Tris, Hydrochloride has also been incorporated in coelomic fluid and in Cortland medium to store unfertilized rainbow trout eggs. Tris, Hydrochloride is also known as Tris(hydroxymethyl)aminomethane hydrochloride, and Tris HCl.