3-Morpholinopropane Sulfonic Acid Mops Biological Buffer CAS# 1132-61-2

Model NO.
DS-CP24
Content
Standard
Usage
Laboratory Reagents, Analytical Reagents, Diagnostic Reagents, Teaching Reagents
Source
Dry Powder
Habit Appellation
Fine Chemicals
Application
Industry, Scientific Research, Health, Environmental Protection
Property
Biochemical Reagent
Molecular Formula
C7h15no4s
Scope
Good′s Buffers
Function
pH Adjustment
Abbreviation
Mops
Molecular Weight
209.26
English Name
3-Morpholinopropane Sulfonic Acid
Transport Package
25kgs Per Drum
Specification
Purity-99.5%
Trademark
DESHENG
Origin
Ezhou, China
HS Code
2934999099
Production Capacity
50tons/Month
Reference Price
$ 27.00 - 36.00

Product Description

3-morpholinopropane sulfonic acid MOPS Biological Buffer CAS# 1132-61-2
3-Morpholinopropane Sulfonic Acid Mops Biological Buffer CAS# 1132-61-2
(
English name ) 3-Morpholinopropane sulfonic acid

( Chinese name ) 3-Morpholinopropane sulfonic acid
( Abbreviation ) MOPS
( CAS# ) 1132-61-2
( M olecular weight ) 209.26
( M olecular  f ormula ) C7H15NO4S
( S torage condition ) room temperature, away from light and moisture
( Molecular structure )
3-Morpholinopropane Sulfonic Acid Mops Biological Buffer CAS# 1132-61-2
(Applicatio n ) Mops is a kind of biological buffer, which is often used to prepare RNA electrophoresis buffer.
(Product advantage) The purity is ≥99%, with good water solubility and stable process, which can ensure that the product appearance is pure white crystal powder.
MOPS   ( 3-( N -morpholino)propanesulfonic acid ) is a   buffer   introduced in the 1960s, one of the twenty   Good's buffers . It is a structural analog to   MES , [1]   and like MES, its structure contains a   morpholine   ring.   HEPES   is a similar   pH   buffering compound that contains a   piperazine   ring. With a   p K a   of 7.20, MOPS is an excellent buffer for many biological systems at near-neutral pH.

Applications

MOPS is frequently used as a   buffering agent   in   biology   and   biochemistry . It has been tested and recommended for   polyacrylamide gel electrophoresis . [2]   Usage above 20 mM in mammalian   cell culture   work is not recommended. [3]   MOPS buffer solutions become discolored (yellow) over time, but reportedly slight discoloration does not significantly affect the buffering characteristics. [4]
 

MOPS Buffer (10X) (0.2 M, pH 7) Preparation and Recipe

MOPS buffer is often used in polyacrylamide gel electrophoresis.
 
To prepare    L   of  MOPS Buffer (10X) (0.2 M, pH 7) :
Change the value in the textbox above to scale the recipe volume
 
Table 1. Required components
Component Amount Concentration
MOPS free acid   (mw:   209.26   g/mol) 41.86   g 0.2   M
Sodium Acetate   (mw:   82.03   g/mol) 4.1   g 0.05   M
Disodium EDTA   (mw:   372.24   g/mol) 3.72   g 0.01   M
  1. Prepare   800   mL   of   dH2O   in a suitable container.
  2. Add   41.86   g   of   MOPS free acid   to the solution.
  3. Add   4.1   g   of   Sodium Acetate   to the solution.
  4. Add   3.72   g   of   Disodium EDTA   to the solution.
  5. Adjust solution to desired pH using NaOH (typical pH = 7)
  6. Add   dH2O   until the volume is   1   L.

 

PNEUTEC.IT, 2023