3-morpholinopropane sulfonic acid MOPS Biological Buffer CAS# 1132-61-2
(
English name
)
3-Morpholinopropane sulfonic acid
(
Chinese name
)
3-Morpholinopropane sulfonic acid
(
Abbreviation
)
MOPS
(
CAS#
)
1132-61-2
(
M
olecular weight
)
209.26
(
M
olecular
f
ormula
)
C7H15NO4S
(
S
torage condition
)
room temperature, away from light and moisture
(
Molecular structure
)
(Applicatio
n
)
Mops is a kind of biological buffer, which is often used to prepare RNA electrophoresis buffer.
(Product advantage)
The purity is ≥99%, with good water solubility and stable process, which can ensure that the product appearance is pure white crystal powder.
MOPS
(
3-(
N
-morpholino)propanesulfonic acid
) is a
buffer
introduced in the 1960s, one of the twenty
Good's buffers
. It is a structural analog to
MES
,
[1]
and like MES, its structure contains a
morpholine
ring.
HEPES
is a similar
pH
buffering compound that contains a
piperazine
ring. With a
p
K
a
of 7.20, MOPS is an excellent buffer for many biological systems at near-neutral pH.
Applications
MOPS is frequently used as a
buffering agent
in
biology
and
biochemistry
. It has been tested and recommended for
polyacrylamide gel electrophoresis
.
[2]
Usage above 20 mM in mammalian
cell culture
work is not recommended.
[3]
MOPS buffer solutions become discolored (yellow) over time, but reportedly slight discoloration does not significantly affect the buffering characteristics.
[4]
MOPS Buffer (10X) (0.2 M, pH 7) Preparation and Recipe
MOPS buffer is often used in polyacrylamide gel electrophoresis.
To prepare
L
of
MOPS Buffer (10X) (0.2 M, pH 7)
:
Change the value in the textbox above to scale the recipe volume
Table 1. Required components
Component
|
Amount
|
Concentration
|
MOPS free acid
(mw:
209.26
g/mol)
|
41.86
g
|
0.2
M
|
Sodium Acetate
(mw:
82.03
g/mol)
|
4.1
g
|
0.05
M
|
Disodium EDTA
(mw:
372.24
g/mol)
|
3.72
g
|
0.01
M
|
-
Prepare
800
mL
of
dH2O
in a suitable container.
-
Add
41.86
g
of
MOPS free acid
to the solution.
-
Add
4.1
g
of
Sodium Acetate
to the solution.
-
Add
3.72
g
of
Disodium EDTA
to the solution.
-
Adjust solution to desired pH using NaOH (typical pH = 7)
-
Add
dH2O
until the volume is
1
L.