Mops Biological Good′ S Buffer CAS# 1132-61-2

Model NO.
DS-CP24
Content
Standard
Usage
Laboratory Reagents, Analytical Reagents, Diagnostic Reagents, Teaching Reagents
Source
Dry Powder
Habit Appellation
Fine Chemicals
Application
Industry, Scientific Research, Health, Environmental Protection
Property
Biochemical Reagent
Molecular Formula
C7h15no4s
Scope
Good′s Buffers
Function
pH Adjustment
English Name
3-Morpholinopropane Sulfonic Acid
Abbreviation
Mops
Transport Package
25kgs Per Drum
Specification
Purity-99.5%
Trademark
DESHENG
Origin
Ezhou, China
HS Code
2934999099
Production Capacity
50tons/Month
Reference Price
$ 27.00 - 36.00

Product Description

3-morpholinopropane sulfonic acid MOPS Biological Buffer CAS# 1132-61-2
Mops Biological Good′ S Buffer CAS# 1132-61-2
(
English name ) 3-Morpholinopropane sulfonic acid

( Chinese name ) 3-Morpholinopropane sulfonic acid
( Abbreviation ) MOPS
( CAS# ) 1132-61-2
( M olecular weight ) 209.26
( M olecular  f ormula ) C7H15NO4S
( S torage condition ) room temperature, away from light and moisture
( Molecular structure )
Mops Biological Good′ S Buffer CAS# 1132-61-2
(Applicatio n ) Mops is a kind of biological buffer, which is often used to prepare RNA electrophoresis buffer.
(Product advantage) The purity is ≥99%, with good water solubility and stable process, which can ensure that the product appearance is pure white crystal powder.
MOPS   ( 3-( N -morpholino)propanesulfonic acid ) is a   buffer   introduced in the 1960s, one of the twenty   Good's buffers . It is a structural analog to   MES , [1]   and like MES, its structure contains a   morpholine   ring.   HEPES   is a similar   pH   buffering compound that contains a   piperazine   ring. With a   p K a   of 7.20, MOPS is an excellent buffer for many biological systems at near-neutral pH.

Applications

MOPS is frequently used as a   buffering agent   in   biology   and   biochemistry . It has been tested and recommended for   polyacrylamide gel electrophoresis . [2]   Usage above 20 mM in mammalian   cell culture   work is not recommended. [3]   MOPS buffer solutions become discolored (yellow) over time, but reportedly slight discoloration does not significantly affect the buffering characteristics. [4]
 

MOPS Buffer (10X) (0.2 M, pH 7) Preparation and Recipe

MOPS buffer is often used in polyacrylamide gel electrophoresis.
 
To prepare    L   of  MOPS Buffer (10X) (0.2 M, pH 7) :
Change the value in the textbox above to scale the recipe volume
 
Table 1. Required components
Component Amount Concentration
MOPS free acid   (mw:   209.26   g/mol) 41.86   g 0.2   M
Sodium Acetate   (mw:   82.03   g/mol) 4.1   g 0.05   M
Disodium EDTA   (mw:   372.24   g/mol) 3.72   g 0.01   M
  1. Prepare   800   mL   of   dH2O   in a suitable container.
  2. Add   41.86   g   of   MOPS free acid   to the solution.
  3. Add   4.1   g   of   Sodium Acetate   to the solution.
  4. Add   3.72   g   of   Disodium EDTA   to the solution.
  5. Adjust solution to desired pH using NaOH (typical pH = 7)
  6. Add   dH2O   until the volume is   1   L.

MOPS is a zwitterionic biological buffer often used in biological and biochemical research. After reviewing several academic papers in which MOPS buffer was studied, we would like to share with you some recommendations and concerns you must have when using this buffer. We hope you find these tips useful!

 

MOPS basic information:

  • CAS Number: 1132-61-2
  • Molecular Weight: 209.3
  • Formula: C 7 H 15 NO 4 S
  • Useful ph range: 6.5 - 7.9
  • pKa (25°C): 7.0 - 7.4
  • Price / Specifications:   Click here

 

What is MOPS recommended for?

  1. For denaturing gel   electrophoresis   of RNA 1
  2. For protein purification in chromatography
  3. To measure absorption during UV/VIS spectrophotometry and to study redox properties using cyclic voltammetry 2
  4. In nitrogenase electron transfer mechanism 3
  5. In the separation of nucleic acids and proteins by   electrophoresis 4
  6. In the control of culture media pH 5 , including cell culture media for yeast, bacteria and mammalian cells.
  7. It has been used as a buffer component of charcoal yeast extract medium 6
  8. It interacts with peptide backbone of bovine serum albumin leading to a net stabilization of the protein 7

 

Which concerns should you have before choosing MOPS for your research?

  • MOPS concentration should be not higher than 20mM when used for mammalian cell culture
  • Although most studies found   little complexation between Mops and metals , some studies   found that interferences may occur due to metal complex formation 8
  • It can modify lipid interactions 9
  • MOPS can influence the thickness and barrier properties of rat endothelial surface layers 10
  • It interacts and forms complexes with DNA 11
  • It can slightly affect mRNA expression of in vitro produced bovine embryos 12
  • Mops interferes with Lowry protein determination
  • It can be oxidized by H 2 O 2 , but since buffer oxidation is slow, no significant impact on biological systems is expected to happen 13
  • MOPS is partly degraded on autoclaving in the presence of glucose

 

Useful tips about MOPS:

  • It does not interfere with Bradford or bicinchoninic acid assays 14

 

Further reading: Why use MOPS?

 

Desheng MOPS Buffer

Desheng Biochem is among the largest producers of MOPS in the world. Our products are shipped daily to top research centers and biotech companies in Europe, America and Asia.

What we offer:

  • Product straight from our manufacturing sites
  • Worldwide shipping to your door
  • Assistance with shipping
  • Small and bulk packages (from grams to tons)
  • International quality standards
  • After-sales service with English speaking staff
Check the specs and price of  Desheng  MOPS

 

PNEUTEC.IT, 2023