3-morpholinopropane sulfonic acid MOPS Biological Buffer CAS# 1132-61-2
(
English name
)
3-Morpholinopropane sulfonic acid
(
Chinese name
)
3-Morpholinopropane sulfonic acid
(
Abbreviation
)
MOPS
(
CAS#
)
1132-61-2
(
M
olecular weight
)
209.26
(
M
olecular
f
ormula
)
C7H15NO4S
(
S
torage condition
)
room temperature, away from light and moisture
(
Molecular structure
)
(Applicatio
n
)
Mops is a kind of biological buffer, which is often used to prepare RNA electrophoresis buffer.
(Product advantage)
The purity is ≥99%, with good water solubility and stable process, which can ensure that the product appearance is pure white crystal powder.
MOPS
(
3-(
N
-morpholino)propanesulfonic acid
) is a
buffer
introduced in the 1960s, one of the twenty
Good's buffers
. It is a structural analog to
MES
,
[1]
and like MES, its structure contains a
morpholine
ring.
HEPES
is a similar
pH
buffering compound that contains a
piperazine
ring. With a
p
K
a
of 7.20, MOPS is an excellent buffer for many biological systems at near-neutral pH.
Applications
MOPS is frequently used as a
buffering agent
in
biology
and
biochemistry
. It has been tested and recommended for
polyacrylamide gel electrophoresis
.
[2]
Usage above 20 mM in mammalian
cell culture
work is not recommended.
[3]
MOPS buffer solutions become discolored (yellow) over time, but reportedly slight discoloration does not significantly affect the buffering characteristics.
[4]
MOPS Buffer (10X) (0.2 M, pH 7) Preparation and Recipe
MOPS buffer is often used in polyacrylamide gel electrophoresis.
To prepare
L
of
MOPS Buffer (10X) (0.2 M, pH 7)
:
Change the value in the textbox above to scale the recipe volume
Table 1. Required components
Component
|
Amount
|
Concentration
|
MOPS free acid
(mw:
209.26
g/mol)
|
41.86
g
|
0.2
M
|
Sodium Acetate
(mw:
82.03
g/mol)
|
4.1
g
|
0.05
M
|
Disodium EDTA
(mw:
372.24
g/mol)
|
3.72
g
|
0.01
M
|
-
Prepare
800
mL
of
dH2O
in a suitable container.
-
Add
41.86
g
of
MOPS free acid
to the solution.
-
Add
4.1
g
of
Sodium Acetate
to the solution.
-
Add
3.72
g
of
Disodium EDTA
to the solution.
-
Adjust solution to desired pH using NaOH (typical pH = 7)
-
Add
dH2O
until the volume is
1
L.
MOPS is a zwitterionic biological buffer often used in biological and biochemical research. After reviewing several academic papers in which MOPS buffer was studied, we would like to share with you some recommendations and concerns you must have when using this buffer. We hope you find these tips useful!
MOPS basic information:
-
CAS Number: 1132-61-2
-
Molecular Weight: 209.3
-
Formula: C
7
H
15
NO
4
S
-
Useful ph range: 6.5 - 7.9
-
pKa (25°C): 7.0 - 7.4
-
Price / Specifications:
Click here
What is MOPS recommended for?
-
For denaturing gel
electrophoresis
of RNA
1
-
For protein purification in chromatography
-
To measure absorption during UV/VIS spectrophotometry and to study redox properties using cyclic voltammetry
2
-
In nitrogenase electron transfer mechanism
3
-
In the separation of nucleic acids and proteins by
electrophoresis
4
-
In the control of culture media pH
5
, including cell culture media for yeast, bacteria and mammalian cells.
-
It has been used as a buffer component of charcoal yeast extract medium
6
-
It interacts with peptide backbone of bovine serum albumin leading to a net stabilization of the protein
7
Which concerns should you have before choosing MOPS for your research?
-
MOPS concentration should be not higher than 20mM when used for mammalian cell culture
-
Although most studies found
little complexation between Mops and metals
, some studies
found that interferences may occur due to metal complex formation
8
-
It can modify lipid interactions
9
-
MOPS can influence the thickness and barrier properties of rat endothelial surface layers
10
-
It interacts and forms complexes with DNA
11
-
It can slightly affect mRNA expression of in vitro produced bovine embryos
12
-
Mops interferes with Lowry protein determination
-
It can be oxidized by H
2
O
2
, but since buffer oxidation is slow, no significant impact on biological systems is expected to happen
13
-
MOPS is partly degraded on autoclaving in the presence of glucose
Useful tips about MOPS:
-
It does not interfere with Bradford or bicinchoninic acid assays
14
Further reading:
Why use MOPS?
Desheng MOPS Buffer
Desheng Biochem is among the largest producers of MOPS in the world. Our products are shipped daily to top research centers and biotech companies in Europe, America and Asia.
What we offer:
-
Product straight from our manufacturing sites
-
Worldwide shipping to your door
-
Assistance with shipping
-
Small and bulk packages (from grams to tons)
-
International quality standards
-
After-sales service with English speaking staff
Check the specs and price of
Desheng
MOPS