Avian Leukemia Virus Antigen Rapid Test (ALV Ag)
Principle
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Immunochromatographic assay
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Intended use
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Veterinary in vitro diagnostic
1) Auxiliary diagnosis on Canine infection for veterinarians in practice.
2) Precautionary diagnosis for home use, point-of-care and early judgement for pet owners.
3) Study use for researchers.
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Detection time
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5 to 10 minutes
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Specime
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Serum, secretions or spleen
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Storage
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4 to 30 degree centigrade
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Shelf life
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24 months
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Package
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10 rapid tests
10 sterile swabs
10 assay buffer
1 package insert
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Advantages
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1) Good sensitivity and specificity
2) Strong R&D and technical support
3) Stable performance.
4) Friendly to end users
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Relative products
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- Canine CPV-CCV Combo Test
- Giardia Antigen Test
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INTENDED USE
The Avian Leukemia Virus Antigen Rapid Test is a lateral flow immunochromatographic assay for the qualitative detection of Avian Leukemia virus antigen (ALV Ag) from bird's serum, plasma, the tissues of lesion or secretions from cloaca.
Assay Time:
5-10 minutes
S
pecimen
:
serum, secretions or spleen
PRINCIPLE
The Avian Leukemia Virus Antigen Rapid Test is based on sandwich lateral flow immunochromatographic assay. The test device has a testing window for the observation of assay running and result reading. The testing window has an invisible T (test) zone and a C (control) zone before running the assay. When the treated sample was applied into the sample hole on the device, the liquid will laterally flow through the surface of the test strip and react with the pre-coated monoclonal antibodies. If there is ALV antigen in the specimen, a visible T line will appear. The C line should always appear after a sample is applied, which indicates a valid result. By this means, the device can accurately indicate the presence of ALV antigen in the specimen.
TEST PROCEDURE
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Collect bird's serum or plasma into a centrifuge tube. Make sure the specimen is clean and transparent. Transfer 3 drops (around 100μL) of the specimen into the assay buffer tube and mix them well. Use the mixture in the assay.
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If the specimen is the tissues of lesion, collect a part of the tissue into the assay buffer. Agitate and crush the tissue and make the tissue mixed as much as possible in the assay buffer. Use the supernatant mixture in the assay.
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If the specimen is secretions from cloaca, collect the bird's cloacal secretions or feces with the cotton swab and insert the swab into the provided assay buffer tube. Agitate it to get efficient sample extraction.
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Take out the test device from the foil pouch and place it horizontally.
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Suck the treated sample extraction from the assay buffer tube and place 3 drops into the sample hole "S" of the test device.
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Interpret the result in 5-10 minutes. Result after 10 minutes is considered as invalid.
COMPANY INTRODUCTION
J&G Biotech Ltd is a privately held company located in London, England. J&G Biotech Ltd and its corporates are engaged in the manufacturing and distribution of in vitro diagnostic products for companion animals, livestock, as well as rapid screening food safety test kits for veterinary drug residues, mycotoxins, and food-borne pathogens. The companion animal rapid test kits have a worldwide distribution under the brand PetX. Some innovative immunoassays are developed to work with reader machines for better service to veterinary practitioners.
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