| Description |
Rabbit monoclonal to beta Actin antibody |
| Protein full name |
Actin, cytoplasmic 1 |
| Synonyms |
ACTB , BRWS1, PS1TP5BP1, Beta-actin, actin beta, Beta CytoskeletalActin, β-Actin |
| Immunogen |
KLH conjugated synthetic peptide corresponding to Mouse beta-Actin |
| Isotype |
IgG1 |
| Purity |
Affinity purification |
| Subcellular location |
Cytoplasm |
| Predicted MW. |
42 kDa |
| Observed MW. |
45 kDa |
| Uniprot ID |
P60710 |
| Applications | Species | Dilution | Positive Sample |
|
WB |
Human, Mouse, Rat |
1: 1000-1: 2000 |
HeLa, Jurkat, MCF-7, PC-3, HepG2 |
|
IHC/IF |
Human, Mouse, Rat |
1: 500-1: 1000 |
spleen, colon cancer, lung, lung cancer |
|
ICC/IF |
Human, Mouse, Rat |
1: 300-1: 1000 |
NIH/3T3, A549, etc. |
Actins are highly conserved proteins that are involved in various types of cell motility and are ubiquitously expressed in all eukaryotic cells.In vertebrates 3 main groups of actin isoforms, alpha, beta and gamma have been identified. The alpha actins are found in muscle tissues and are a major constituent of the contractile apparatus. The beta and gamma actins coexist in most cell types as components of the cytoskeleton and as mediators of internal cell motility.
| Storage | Store at -20 ºC for one year. Avoid repeated freeze/ thaw cycles. |
| Storage Buffer | PBS with 0.02% sodium-azide, 100 μg/ml BSA and 50% glycerol. |