Description |
MYC Tag ( EQKLISEEDL Tag ) mouse monoclonal antibody |
Synonyms |
c-myc, Myc, MYC, Myc Epitope Tag, Myc tag, MYC tag, EQKLISEEDL, Tag |
Immunogen |
Synthetic peptide : EQKLISEEDL |
Isotype |
IgG |
Purity |
Affinity purification |
Applications | Dilution | Positive Sample |
WB |
1: 500-1: 1000 |
MYC-Tag fusion protein |
The myc-tag is a short synthetic polypeptide sequence derived from c-myc protein that can be added to recombinant proteins to enable isolation and study when an antibody is not available. This antibody recognizes the MYC tag EQKLISEEDL. Using recombinant DNA technology, the myc tag can be fused to the protein and then an antibody against the myc tag can be used to probe, including purification using chromatography, tracking the protein in localization studies using immunofluorescence, or quantifying levels using Western Blot. The short polypeptide sequence can be fused to the N-terminus or the C-terminus of any protein without influencing function, although it is advised to avoid fusing it to a secretory signal.
Storage | Store at -20 ºC for one year. Avoid repeated freeze/ thaw cycles. |
Storage Buffer | PBS with 0.02% sodium-azide, 100 μg/ml BSA and 50% glycerol. |