Main applications of Tris
1,Used in biological buffers
In experimental research and analysis, Tris is the buffer used more after phosphate buffer, usually together with acid to ensure the pH value of the reaction system, generally use Tris HCL buffer, Tris acetate buffer, etc. In the past, in vitro diagnostic tests, phosphate buffer will be applied, but because of the involvement of calcium and enzymes, it is easy to generate precipitation, and then choose to use Tris, so that it will not precipitate, but also to ensure the buffering effect.
2,For protein purification
In experimental research, the molecular mechanism of life activities and disease occurrence can be further clarified by protein, so at this time protein extraction is one of the basic and key links. Because it can play a role in maintaining the protein PH value, to ensure that the basic spatial structure of the protein does not change, and has the characteristics of preventing denaturation, so it has become the main buffer.